5XA0

Crystal structure of inositol 1,4,5-trisphosphate receptor cytosolic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-26
Detector	RAYONIX MX225HE
Wavelength(s)	1.00
Spacegroup name	P 42
Unit cell lengths	128.164, 128.164, 369.486
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.807 - 5.812
R-factor	0.2861
Rwork	0.278
R-free	0.34170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5X9Z chain A and 3UJ4 chain A
RMSD bond length	0.014
RMSD bond angle	1.779
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	6.020
High resolution limit [Å]	5.810	5.812
Rmerge		0.570
Number of reflections	16347	1614
<I/σ(I)>	9.27	2.22
Completeness [%]	99.8	100
Redundancy	10.5	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	lithium sulfate