5X9S

Crystal structure of fully modified H-Ras-GppNHp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-03
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	88.475, 88.475, 134.047
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.680 - 2.500
R-factor	0.18603
Rwork	0.184
R-free	0.23605
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k8y
RMSD bond length	0.008
RMSD bond angle	1.319
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	7216
<I/σ(I)>	21.6
Completeness [%]	100.0	100
Redundancy	10.7	11
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Ca acetate, 20%(w/v) PEG8000, 0.1M MES (pH6.0)