5X9S
Crystal structure of fully modified H-Ras-GppNHp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-03 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.475, 88.475, 134.047 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.680 - 2.500 |
| R-factor | 0.18603 |
| Rwork | 0.184 |
| R-free | 0.23605 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k8y |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.319 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 7216 | |
| <I/σ(I)> | 21.6 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 | 11 |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Ca acetate, 20%(w/v) PEG8000, 0.1M MES (pH6.0) |
