5X9L
Recombinant thaumatin I at 0.9 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-07-08 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.702, 57.702, 149.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.900 |
| Rwork | 0.117 |
| R-free | 0.12900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3al7 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.040 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.920 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.068 | 0.498 |
| Number of reflections | 187168 | |
| <I/σ(I)> | 67.39 | 4.13 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.6 | 6.6 |
| CC(1/2) | 0.998 | 0.901 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M ADA, 0.75 M sodium potassium tartrate |
