5X9H
Crystal structure of the Mg2+ channel MgtE in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.330, 85.651, 156.558 |
| Unit cell angles | 90.00, 100.02, 90.00 |
Refinement procedure
| Resolution | 50.329 - 3.598 |
| R-factor | 0.2533 |
| Rwork | 0.250 |
| R-free | 0.28320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.519 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.329 |
| High resolution limit [Å] | 3.598 |
| Number of reflections | 20361 |
| <I/σ(I)> | 15.09 |
| Completeness [%] | 98.5 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 9% PEG 4000, 0.2 M MgCl2, and 0.05 M MES pH 6.5, 10 mM ATP |
