5X8W
Crystal Structure of the mutant Human ROR gamma Ligand Binding Domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-01 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.740, 61.740, 155.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.400 - 2.300 |
| R-factor | 0.21306 |
| Rwork | 0.212 |
| R-free | 0.22960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l0l |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.928 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.900 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.104 | 0.494 |
| Number of reflections | 14167 | |
| <I/σ(I)> | 7.1 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.9 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M Sodium acetate pH8.0, 4M ammonium acetate |
