5X8V

Y92H mutant of thermus thermophilus HB8 thymidylate kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-05-17
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	46.540, 47.052, 151.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 1.660
R-factor	0.1728
Rwork	0.172
R-free	0.19836
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.872
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.010
High resolution limit [Å]	1.660
Number of reflections	39038
<I/σ(I)>	20.7
Completeness [%]	97.8
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	0.1M Sodium Malonate, 12% PEG 3350