5X7Y
Crystal Structure of the Dog Lipocalin Allergen Can f 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 |
| Unit cell lengths | 96.604, 96.604, 85.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.600 - 2.350 |
| R-factor | 0.20607 |
| Rwork | 0.203 |
| R-free | 0.26882 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gm6 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.822 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.600 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.101 | |
| Rpim | 0.028 | |
| Number of reflections | 32931 | 1615 |
| <I/σ(I)> | 52.2 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 14.3 |
| CC(1/2) | 0.952 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.0 M ammonium dihydrogenphosphate, 20% (w/v) PEG3350 |
