5X7S
Crystal structure of Paenibacillus sp. 598K alpha-1,6-glucosyltransferase, terbium derivative
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-11-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.64883,1.64955,1.60679 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 182.815, 270.920, 133.341 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 151.540 - 2.400 |
| R-factor | 0.18727 |
| Rwork | 0.185 |
| R-free | 0.23871 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.661 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.115 | 0.861 |
| Number of reflections | 128840 | 6364 |
| <I/σ(I)> | 29.4 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.2 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 293 | 1.5 M magnesium sulfate, 0.1 M MES buffer, 1 mM terbium chloride |
