5X7M

Crystal structure of meso-diaminopimelate decarboxylase (DAPDC) from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 2
Unit cell lengths	114.650, 92.013, 94.753
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.708 - 2.400
R-factor	0.1897
Rwork	0.186
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hkv
RMSD bond length	0.014
RMSD bond angle	1.725
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.760	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	36222
<I/σ(I)>	26.88
Completeness [%]	96.0
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Sodium citrate tribasic, Sodium cacodylate