5X7L
Structure of TsrD from Streptomyces laurentii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2016-04-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 54.232, 54.232, 199.952 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.420 - 1.220 |
| R-factor | 0.1514 |
| Rwork | 0.151 |
| R-free | 0.16631 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.978 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.220 |
| Number of reflections | 102592 |
| <I/σ(I)> | 38.3 |
| Completeness [%] | 99.6 |
| Redundancy | 18.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, and 30% v/v 2-Propanol |
