5X6U

Crystal structure of human heteropentameric complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-08
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.90000
Spacegroup name	P 41 2 2
Unit cell lengths	133.038, 133.038, 75.147
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	133.040 - 2.400
R-factor	0.188
Rwork	0.186
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vet 3msh
RMSD bond length	0.010
RMSD bond angle	1.180
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	133.040	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	26934
<I/σ(I)>	23.9
Completeness [%]	100.0
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Tris-HCl, 2-propanol, 1,6-hexandiol, 1,4-butandiol, 1-butanol, 1,3-propanediol, PEG 100, PEG 3350, 2-Methyl-2,4-pentanediol