Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979065 |
| Spacegroup name | P 31 |
| Unit cell lengths | 119.464, 119.464, 93.067 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.937 - 3.200 |
| R-factor | 0.2125 |
| Rwork | 0.210 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kmp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.231 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.095 | 0.556 |
| Rpim | 0.046 | 0.257 |
| Number of reflections | 24244 | 2446 |
| <I/σ(I)> | 22.11 | 4.55 |
| Completeness [%] | 98.8 | 100 |
| Redundancy | 5.5 | 5.7 |
| CC(1/2) | 0.929 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M Bis-tris, pH 6.5, 0.2M LiNO3, 8% PEG 3350, 5% glycerol |
