5X6E

Crystal structure of PrfA-DNA binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	65.017, 96.590, 370.528
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.466 - 2.990
R-factor	0.2515
Rwork	0.249
R-free	0.29970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.278
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	96.620
High resolution limit [Å]	2.990
Number of reflections	46496
<I/σ(I)>	8.7
Completeness [%]	88.8
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Tris, 27% PEG3350, 0.2M Li2SO4, pH 8.5