5X6D

Crystal structure of PrfA-DNA binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979100
Spacegroup name	P 21 21 21
Unit cell lengths	64.820, 96.443, 371.911
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.827 - 2.940
R-factor	0.2158
Rwork	0.213
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bgc
RMSD bond length	0.008
RMSD bond angle	1.310
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	96.440
High resolution limit [Å]	2.930
Number of reflections	50839
<I/σ(I)>	13.3
Completeness [%]	99.1
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Bis-Tris, 15% PEG3350, 0.16M MgCl2, pH 6.5