5X60

Crystal structure of LSD1-CoREST in complex with peptide 9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-02
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	122.005, 180.359, 233.144
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.690
R-factor	0.22169
Rwork	0.221
R-free	0.23626
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v1d
RMSD bond length	0.007
RMSD bond angle	1.077
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.730
High resolution limit [Å]	2.680	2.680
Rmerge	0.090	0.848
Number of reflections	71152	3551
<I/σ(I)>	21.9	2.7
Completeness [%]	99.4	100
Redundancy	7.4	7.6
CC(1/2)		0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M N-(2-acetamido)iminodiacetic acid, 1.23 M potassium sodium tartrate tetrahydrate.