5X51

RNA Polymerase II from Komagataella Pastoris (Type-3 crystal)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-28
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	148.770, 158.050, 254.960
Unit cell angles	90.00, 92.54, 90.00

Refinement procedure

Resolution	33.267 - 6.996
R-factor	0.3372
Rwork	0.338
R-free	0.33510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.819
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_2614)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.267	33.267	7.410
High resolution limit [Å]	7.000	19.490	7.000
Rmerge	0.265	0.069	1.146
Rmeas	0.309	0.081	1.335
Total number of observations	70891
Number of reflections	18745	839	2925
<I/σ(I)>	6.3	16.22	1.54
Completeness [%]	99.1	91	98.2
Redundancy	3.782	3.426	3.777
CC(1/2)	0.962	0.994	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M sodium citrate, 10% PGA-LM, 0.5% dextran sulfate sodium salt, 15 mM Tris, and 35 mM Hepes