5X4S

Structure of the N-terminal domain (NTD)of SARS-CoV spike protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1.039
Spacegroup name	P 61 2 2
Unit cell lengths	73.291, 73.291, 240.263
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.312 - 2.200
R-factor	0.2535
Rwork	0.252
R-free	0.28240
RMSD bond length	0.003
RMSD bond angle	0.742
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	20329	1980
<I/σ(I)>	14.6	5.9
Completeness [%]	99.4	100
Redundancy	11.1	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	1.3 M Na/K hydrogen phosphate