5X4L

Crystal structure of the UBX domain of human UBXD7 in complex with p97 N domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9777
Spacegroup name	P 21 21 21
Unit cell lengths	63.046, 84.822, 109.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.920 - 2.402
R-factor	0.1874
Rwork	0.185
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qq7
RMSD bond length	0.009
RMSD bond angle	1.265
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.920
High resolution limit [Å]	2.400
Rmerge	0.109
Rpim	0.031
Number of reflections	23521
<I/σ(I)>	27
Completeness [%]	100.0
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	0.2M Calcium acetate hydrate pH7.5, 20% v/v PEG 3350