5X42
Structure of DotL(590-659)-DotN derived from Legionella pneumophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.2828 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.683, 72.220, 170.435 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.580 - 1.800 |
R-factor | 0.2121 |
Rwork | 0.211 |
R-free | 0.25980 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.840 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.064 | 0.048 | 0.272 |
Rmeas | 0.067 | 0.050 | 0.313 |
Rpim | 0.019 | 0.014 | 0.149 |
Number of reflections | 104008 | ||
<I/σ(I)> | 14.9 | ||
Completeness [%] | 97.0 | 98.6 | 91.2 |
Redundancy | 8.8 | 12.7 | 3.4 |
CC(1/2) | 0.998 | 0.416 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 100mM sodium malonate (pH 4.0), 10%(v/v) PEG3350, 20%(v/v) glycerol |