5X41

3.5A resolution structure of a cobalt energy-coupling factor transporter using LCP method-CbiMQO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-12-15
Detector	MAR555 FLAT PANEL
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	96.159, 135.040, 204.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.962 - 3.470
R-factor	0.3005
Rwork	0.299
R-free	0.33530
RMSD bond length	0.015
RMSD bond angle	3.174
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.470
Rmerge	0.167
Number of reflections	34336
<I/σ(I)>	10.3
Completeness [%]	100.0
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	0.1M NaCl, 35-48% PEG 400, 0.1M MES, PH 6.5