5X2W

Crystal structure of Pseudomonas putida methionine gamma-lyase wild type with L-methionine intermediates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-03-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	154.635, 152.311, 80.487
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.19797
Rwork	0.195
R-free	0.24635
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o7c
RMSD bond length	0.008
RMSD bond angle	1.324
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.169	0.435
Number of reflections	53025
<I/σ(I)>	8.8
Completeness [%]	99.8	100
Redundancy	7.2	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Na-K phosphate buffer, 6-10 % PEG 6000, 0.25 M ammonium sulfate. 0.5 mM PLP