5X2L
Crystal Structure of Human Serine Racemase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-02 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 80.120, 112.590, 88.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.062 - 1.806 |
R-factor | 0.2221 |
Rwork | 0.220 |
R-free | 0.25810 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.050 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.062 |
High resolution limit [Å] | 1.806 |
Number of reflections | 73388 |
<I/σ(I)> | 9.1 |
Completeness [%] | 100.0 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% PEG 8000, 5mM MgCl2, 10% Ethylene Glycol, 0.1M bis-Tris pH6.0 |