5X2I
Polygalacturonate Lyase by Fusing with a Self-assembling Amphipathic Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2015-12-26 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.094, 51.212, 51.296 |
| Unit cell angles | 67.51, 74.93, 78.75 |
Refinement procedure
| Resolution | 28.427 - 2.050 |
| R-factor | 0.17709 |
| Rwork | 0.176 |
| R-free | 0.19952 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1bn8 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.703 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.427 |
| High resolution limit [Å] | 1.860 |
| Number of reflections | 18202 |
| <I/σ(I)> | 12.1 |
| Completeness [%] | 91.8 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 293 | 0.2 M sodium bromide, 20 mM Glycine-NaOH, 20 % polyethylene glycol 3350, 7 mM calcium chloride, 20 % glycerol |
