5X27

Crystal structure of EGFR 696-1022 L858R in complex with SKLB(5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97923
Spacegroup name	I 2 3
Unit cell lengths	145.384, 145.384, 145.384
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.974 - 2.952
R-factor	0.205
Rwork	0.203
R-free	0.23840
RMSD bond length	0.014
RMSD bond angle	1.339
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.950
Number of reflections	10571
<I/σ(I)>	16.6
Completeness [%]	96.4
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	0.1M Hepes pH 7.8, 40% PEG 400, 0.15M NaCl, 5mM tris(2-carboxyethyl)-phosphine (TCEP)