5X26

Crystal structure of EGFR 696-1022 L858R in complex with SKLB(3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97923
Spacegroup name	I 2 3
Unit cell lengths	145.278, 145.278, 145.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.827 - 2.951
R-factor	0.1969
Rwork	0.196
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2itv
RMSD bond length	0.014
RMSD bond angle	1.277
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.950
Number of reflections	10731
<I/σ(I)>	14.6
Completeness [%]	98.2
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Hepes pH7.8, 40% PEG400, 0.15M NaCl, 5mM tris(2-carboxyethyl)-phosphine (TCEP)