5X1H

Structure of Legionella pneumophila DotN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-24
Detector	ADSC QUANTUM 210
Wavelength(s)	1.2828
Spacegroup name	P 65 2 2
Unit cell lengths	155.357, 155.357, 527.711
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.934 - 3.005
R-factor	0.2572
Rwork	0.256
R-free	0.29730
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.408
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	3.000	8.130	3.000
Rmerge	0.104	0.071	0.317
Rmeas	0.107	0.072	0.406
Rpim	0.023	0.013	0.249
Total number of observations	777894
Number of reflections	109241
<I/σ(I)>	14.5
Completeness [%]	85.4	98.2	60.9
Redundancy	12	27.1	1.8
CC(1/2)		0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	600mM sodium citrate and 100mM sodium cacodylate (pH 6.5)