5X1C

Crystal Structure of Human CRMP-2 without C-terminal Tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-13
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	126.687, 126.687, 148.219
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.263 - 2.101
R-factor	0.1793
Rwork	0.177
R-free	0.20160
RMSD bond length	0.009
RMSD bond angle	1.199
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	70654
<I/σ(I)>	3.5
Completeness [%]	100.0
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	ammonium sulfate, Tris pH 7.0