5X0J
Free serine kinase (E30Q mutant) in complex with phosphoserine and AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2016-02-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.824, 43.400, 157.545 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.430 |
R-factor | 0.18134 |
Rwork | 0.180 |
R-free | 0.19827 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5x0e |
RMSD bond length | 0.007 |
RMSD bond angle | 1.369 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.430 |
Number of reflections | 45510 |
<I/σ(I)> | 21.7 |
Completeness [%] | 97.5 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.7 | 293 | PEG 3350, serine |