5X0E
Free serine kinase (E30A mutant) in complex with phosphoserine and AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-13 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.100 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.667, 43.224, 157.519 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.18298 |
Rwork | 0.181 |
R-free | 0.22188 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vk1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.396 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 16587 |
<I/σ(I)> | 24.3 |
Completeness [%] | 95.9 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 293 | PEG 3350, serine |