5X06

DNA replication regulation protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	103.15
Detector technology	CCD
Collection date	2014-04-24
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	102.112, 149.542, 200.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.593 - 3.237
R-factor	0.2277
Rwork	0.226
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2POL/3BOS
RMSD bond length	0.002
RMSD bond angle	0.565
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.290
High resolution limit [Å]	3.230	3.230
Number of reflections	49691
<I/σ(I)>	36.2	4.2
Completeness [%]	98.7	98.4
Redundancy	6.6	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1M Tris-Cl pH 7.0, 10.5% PEG 400, 0.2M Calcium chloride, 10mM Trimethylamine-HCl, 1mM ADP, 5mM Magnesium chloride