5X03

Crystal structure of the C-terminal domain of Bacillus subtilis GabR reveals a closed conformation by the binding of gamma-aminobutyric acid, inducing the transcriptional activation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-20
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 41
Unit cell lengths	118.497, 118.497, 75.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.472 - 2.000
R-factor	0.1741
Rwork	0.173
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.126
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	69052
<I/σ(I)>	14.6
Completeness [%]	96.5
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1M HEPES pH 7.5, 1.5%(w/v) polyethylene glycol 8000