5WZI

Structure of APUM23-GGAGUUGACGG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97916
Spacegroup name	P 65
Unit cell lengths	190.497, 190.497, 45.727
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.993 - 2.750
R-factor	0.1914
Rwork	0.189
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wzg
RMSD bond length	0.008
RMSD bond angle	0.932
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.800
High resolution limit [Å]	2.750	7.450	2.750
Rmerge	0.143	0.067	0.777
Rmeas	0.150	0.070	0.812
Rpim	0.043	0.020	0.234
Number of reflections	25175
<I/σ(I)>	6.5
Completeness [%]	100.0	99.9	100
Redundancy	11.9	11.3	11.8
CC(1/2)		0.997	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.15 M DL-Malic acid (pH 7.0), 20% w/v Polyethylene glycol 3350