5WZH

Structure of APUM23-GGAAUUGACGG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97916
Spacegroup name	P 65
Unit cell lengths	188.409, 188.409, 45.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.884 - 2.509
R-factor	0.1891
Rwork	0.187
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wzg
RMSD bond length	0.007
RMSD bond angle	0.911
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.115	0.042	0.763
Rmeas	0.121	0.044	0.798
Rpim	0.034	0.012	0.227
Total number of observations	392238
Number of reflections	31090
<I/σ(I)>	7.1
Completeness [%]	96.5	94	97.3
Redundancy	12.6	12.5	12.8
CC(1/2)		0.998	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.15 M DL-Malic acid (pH 7.0), 20% w/v Polyethylene glycol 3350