5WZ1
Crystal structure of Zika virus NS5 methyltransferase bound to S-adenosyl-L-methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.549, 86.847, 107.556 |
| Unit cell angles | 90.00, 97.39, 90.00 |
Refinement procedure
| Resolution | 45.447 - 2.507 |
| R-factor | 0.2468 |
| Rwork | 0.235 |
| R-free | 0.25680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l9k |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.719 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.195 |
| Number of reflections | 66444 |
| <I/σ(I)> | 8.075 |
| Completeness [%] | 98.6 |
| Redundancy | 5.7 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2 M magnesium chloride, 0.1 M HEPES pH 7.0, 20 % w/v PEG 6000 |
