5WYL

Crystal structure of Chaetomium thermophilum Utp10 N-terminal domain in complex with Utp17 C-terminal helices

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 41 21 2
Unit cell lengths	98.309, 98.309, 235.653
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.010 - 2.638
R-factor	0.215
Rwork	0.212
R-free	0.26670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wy4
RMSD bond length	0.009
RMSD bond angle	1.029
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.11rc1_2513: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.154	49.154	2.800
High resolution limit [Å]	2.640	7.820	2.640
Rmerge	0.077	0.078	0.185
Rmeas	0.078	0.080	0.188
Total number of observations	943385
Number of reflections	34951	1508	5518
<I/σ(I)>	35.46	50.14	15.87
Completeness [%]	100.0	99.3	99.9
Redundancy	26.992
CC(1/2)	0.999	0.997	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M Hepes-Na, 20% w/v PEG 8000