5WYD
Structural of Pseudomonas aeruginosa DspI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97022 |
| Spacegroup name | P 31 |
| Unit cell lengths | 83.309, 83.309, 207.547 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.657 - 2.101 |
| R-factor | 0.2303 |
| Rwork | 0.227 |
| R-free | 0.27620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5WYC |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.375 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1-2155_1692: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 90344 | 4323 |
| <I/σ(I)> | 15.44 | 1.9 |
| Completeness [%] | 96.2 | 92.1 |
| Redundancy | 5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.5M (NH4)2SO4, 0.1M CH3COONa pH 4.5 |
