5WYB

Structure of Pseudomonas aeruginosa DspI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-12-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97776
Spacegroup name	P 63 2 2
Unit cell lengths	125.262, 125.262, 72.651
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.758 - 2.250
R-factor	0.2337
Rwork	0.230
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fzw
RMSD bond length	0.015
RMSD bond angle	1.296
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1-2155_1692: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.758	2.310
High resolution limit [Å]	2.250	2.250
Number of reflections	16394	1330
<I/σ(I)>	19
Completeness [%]	100.0	99.9
Redundancy	20.5	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	10% isopropanol, 5% MPD, 0.1M CH3COONa pH 5.0 0.5M (NH4)2SO4, 0.1M CH3COONa pH 4.5