5WY4

Crystal structure of Chaetomium thermophilum Utp10 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	81.888, 70.458, 93.728
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.908 - 2.499
R-factor	0.2263
Rwork	0.222
R-free	0.26380
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.979
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((1.11rc1_2513: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.781	2.500
Rmerge	0.103	0.062	0.461
Total number of observations	168375
Number of reflections	35781
<I/σ(I)>	15.9
Completeness [%]	98.6	85	100
Redundancy	4.7	3.6	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1 M sodium citrate, 1.0 M ammonium dihydrogen phosphate