5WY1

Crystal structure of mouse DNA methyltransferase 1 (T1505A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-17
Detector	RAYONIX MX300HE
Wavelength(s)	0.900
Spacegroup name	P 21 21 2
Unit cell lengths	134.998, 97.804, 130.319
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.270
R-factor	0.2061
Rwork	0.203
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3av4
RMSD bond length	0.010
RMSD bond angle	1.415
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.320
High resolution limit [Å]	3.260	8.830	3.260
Rmerge	0.190	0.049	0.748
Rmeas	0.219
Rpim	0.106
Number of reflections	26720
<I/σ(I)>	5.4
Completeness [%]	97.5	92.7	96.4
Redundancy	4.4	4.1	4.4
CC(1/2)		0.992	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	PEG 3350, NaCl, TCEP, Na-citrate