5WXJ
Apo EarP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2015-09-30 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.622, 195.774, 46.396 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.361 - 1.850 |
| R-factor | 0.1592 |
| Rwork | 0.158 |
| R-free | 0.19770 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.363 |
| Data scaling software | Aimless |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.940 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 77019 | |
| <I/σ(I)> | 11.5 | 2 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.4 | 7.1 |
| CC(1/2) | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M Hepes-NaOH buffer (pH 6.8-7.5), 20% PEG3350, and 0.2 M ammonium sulfate |
