5WWZ
Crystal structure of the KH2 domain of human RNA-binding E3 ubiquitin-protein ligase MEX-3C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 83.321, 83.321, 78.959 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.262 - 2.500 |
| R-factor | 0.2284 |
| Rwork | 0.226 |
| R-free | 0.26980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5www |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.430 |
| Data reduction software | MOSFLM |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.082 | 0.039 | 0.563 |
| Rmeas | 0.089 | 0.042 | 0.609 |
| Rpim | 0.033 | 0.016 | 0.230 |
| Total number of observations | 70047 | ||
| Number of reflections | 10139 | ||
| <I/σ(I)> | 4.4 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 6.9 | 6.4 | 6.9 |
| CC(1/2) | 0.999 | 0.888 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1.6M Ammonium sulfate |
