5WWX

Crystal structure of the KH2 domain of human RNA-binding E3 ubiquitin-protein ligase MEX-3C complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	74.611, 74.611, 32.535
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.367 - 2.000
R-factor	0.1724
Rwork	0.170
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5www
RMSD bond length	0.008
RMSD bond angle	0.923
Data reduction software	MOSFLM
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.108	0.048	0.534
Number of reflections	6609
<I/σ(I)>	3.6
Completeness [%]	99.6	98	99.8
Redundancy	7.6	7	7.9
CC(1/2)		0.998	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.04M Magnesium acetate tetrahydrate, 0.05M Sodium cacodylate trihydrate pH 6.0, 30% v/v (+/-)-2-Methyl-2,4-pentanediol