5WWV
Crystal structure of porcine kidney D-amino acid oxidase mutant (I230A/R283G)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-19 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.10 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 170.064, 273.950, 136.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.730 - 3.200 |
| R-factor | 0.14979 |
| Rwork | 0.145 |
| R-free | 0.24212 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wgt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.687 |
| Data reduction software | XDS (June 17, 2015) |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | MOLREP (11.2.08) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 144.490 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.111 | 0.603 |
| Number of reflections | 52954 | |
| <I/σ(I)> | 13.3 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 10% PEG 3350, 15% MPD, 200 mM Lithium sulfate, 100 mM Bis-Tris-HCl pH 6.5 |
