5WWU

Crystal Structure of HLA-A*2402 in complex with 2009 pandemic influenza A(H1N1) virus and avian influenza A(H5N1) virus-derived peptide H1-25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-10
Detector	SDMS
Wavelength(s)	0.97774
Spacegroup name	P 21 21 21
Unit cell lengths	71.752, 79.099, 88.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.506 - 2.794
R-factor	0.2275
Rwork	0.225
R-free	0.28420
RMSD bond length	0.003
RMSD bond angle	0.703
Data reduction software	CNS
Data scaling software	CNS
Phasing software	CNS
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.794
Number of reflections	138842
<I/σ(I)>	35.2
Completeness [%]	94.5
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4000