5WWS
Crystal structure of human NSun6/tRNA/SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 205.236, 170.262, 76.883 |
| Unit cell angles | 90.00, 108.63, 90.00 |
Refinement procedure
| Resolution | 42.702 - 3.247 |
| R-factor | 0.1949 |
| Rwork | 0.193 |
| R-free | 0.22210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wwr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.783 |
| Data reduction software | SCALEPACK |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.247 |
| Number of reflections | 39477 |
| <I/σ(I)> | 26.9 |
| Completeness [%] | 94.6 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.8 | 289 | 0.1 M SPG, 26% (w/v) PEG 1500, 50 mM NaF |
