5WWR

Crystal structure of human NSun6/tRNA/SFG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97776
Spacegroup name	C 1 2 1
Unit cell lengths	206.908, 170.478, 76.999
Unit cell angles	90.00, 108.55, 90.00

Refinement procedure

Resolution	42.892 - 3.096
R-factor	0.1973
Rwork	0.195
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wwq 2du3
RMSD bond length	0.003
RMSD bond angle	0.593
Data reduction software	SCALEPACK
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.096
Number of reflections	45890
<I/σ(I)>	19.7
Completeness [%]	98.2
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	289	0.1 M SPG, 25% (w/v) PEG 1500, 50 mM NaF