5WVN

Crystal structure of MBS-BaeS fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2015-06-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 43 21 2
Unit cell lengths	119.084, 119.084, 147.011
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.680 - 2.800
R-factor	0.23
Rwork	0.228
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.148
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.684	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	25337
<I/σ(I)>	39.3	3.2
Completeness [%]	99.9	99.8
Redundancy	10.1	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	Citric Acid: NaOH , Ammonium Sulfate