5WV7
Crystal structure of d(CCGGGGTACCCCGG)2 at 1.4A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97372 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 41.803, 41.803, 89.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.063 - 1.406 |
| R-factor | 0.2229 |
| Rwork | 0.222 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v9d |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.199 |
| Data reduction software | XDS (GPLv2) |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.060 | 28.060 | 1.430 |
| High resolution limit [Å] | 1.400 | 7.430 | 1.400 |
| Rmerge | 0.081 | 0.038 | 0.496 |
| Rmeas | 0.083 | 0.039 | 4.751 |
| Rpim | 0.016 | 0.008 | 1.182 |
| Total number of observations | 437650 | 2392 | 12306 |
| Number of reflections | 16170 | ||
| <I/σ(I)> | 16.9 | 45.3 | 0.3 |
| Completeness [%] | 99.8 | 85.3 | 98 |
| Redundancy | 27.1 | 19 | 15.3 |
| CC(1/2) | 1.000 | 0.999 | 0.293 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1mM DNA, 50mM sodium cacodylate trihydrate buffer, 1mM Bacl2, 5mM spermine, 50% MPD |
