5WUX
TNFalpha-certolizumab Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-16 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.592, 207.219, 112.631 |
| Unit cell angles | 90.00, 118.81, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.2281 |
| Rwork | 0.226 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.278 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 63355 |
| <I/σ(I)> | 19.5 |
| Completeness [%] | 95.2 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M 3-(cyclohexylamino)-1-propanesulfonic acid pH10.2, 0.2M lithium sulfate, 1.5M ammonium sulfate |
