5WUW
Serratia marcescens short-chain dehydrogenase/reductase F98L/F202L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2016-10-21 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 84.012, 84.012, 115.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.900 |
| R-factor | 0.17358 |
| Rwork | 0.172 |
| R-free | 0.21174 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zgw |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.990 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 33651 |
| <I/σ(I)> | 10.5 |
| Completeness [%] | 92.3 |
| Redundancy | 26.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 50 mM HEPES buffer (pH 7.0), 40% (v/v) tacsimate (pH 7.0), 2 mM spermine, and 2 mM hexamine cobalt (III) chloride |
